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KMID : 0613820120220060778
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Journal of Life Science
2012 Volume.22 No. 6 p.778 ~ p.787
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The Reverse Effect of Salicylic Acid on Cd-induced Growth, Chlorophyll, and Rubisco/Rubisco Activase in Tobacco
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Wang Yu-Shan
Roh Kwang-Soo
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Abstract
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The influence of salicylic acid (SA) on growth, chlorophyll, and rubisco/rubisco activase and effect of denaturator on rubisco/rubisco activase activity were studied in tobacco plants grown in vitro with cadmium (Cd) treatment. In order to find out the optimum concentration of SA, tobacco plants treated with 10^-6 mM - 10² mM of SA were grown in MS medium for 9 weeks, respectively. The most pronounced effect on in vitro growth was found at 10^-4 mM SA. Among the control (not treated with Cd and SA), SA, Cd, and Cd + SA, the growth and content of chlorophyll were in the sequence of Cd < Cd + SA < control < SA, and significantly higher at SA compared with others. Similar results were also observed in the content and activity of rubisco and rubisco activase. These data suggest that inhibitory effect by Cd was reversed by SA. These results also indicate that SA has a positive effect on Cd. The effect of denaturants on rubisco activity showed in the sequence of Cd < Cd + SA < control < SA. Rubisco activity was promoted by L-cysteine and β-mercaptoethanol, not by urea, thiourea, and guanidium-HCl. These data suggest that urea, thiourea, and guanidium-HCl are able to act as denaturator, and L-cysteine and β-mercaptoethanol are not. None of the five denaturants affected the activity of rubisco activase.
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KEYWORD
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Cadmium, denaturants, rubisco, rubisco activase, salicylic acid
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